电离辐射通过转化生长因子-β-介导的上皮-间质匹配来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：深入探讨紫外光是否可通过转化细胞因子-β（TGF-β）-介导的视网膜-间质转换 (EMT)来促进肝细胞的肆虐迁移。使用总量2Gy(60)Coγ支线紫外线称做人类器官的6种肝细胞，历史纪录与EMT相关的变化，这还包括分别并用显微镜电子技术，细胞内质印迹工具，免疫荧光电子技术，样试验和Transwell小室试验来观察并监测细胞组织形态，EMT标记，肆虐迁移能力也等。选用酶联免疫吸附法律条文监测这些肝细胞里TGF-β细胞内水平，并用特别抑制剂SB431542来指标TGF-β频谱路里在紫外光EMT里的作用。经过总量为2Gy紫外线的肝细胞里存在间叶细胞的表达，与假紫外线组相比其视网膜标记减少，间叶细胞标记缩减，同时其肆虐移转到能力也增强，TGF-β细胞内水平也提高。全面性找到由A549紫外光游离的EMT可通过对TGF-β频谱抑制发生逆转。这些结果表明TGF-β介导的EMT在紫外光游离增强肝细胞肆虐移转到能力也里起着关键作用。

撰稿： lizexiu